|
KMID : 0380420120360020066
|
|
Journal of Prventive Veterinary Medicine
2012 Volume.36 No. 2 p.66 ~ p.74
|
|
|
Efficacy of specific immunoglobulim egg yolk (IgY) against enterotoxigenic Escherichia coli K99, Salmonella Typhimurium and Salmonella Choleraesuis
|
|
Kim Bo-Mi
Hong Chong-Hae
Hahn Tae-Wook
|
|
|
Abstract
|
|
|
|
Egg yolk immunoglobulin (IgY) is the antibody in egg yolk and can be produced in egg yolk by immunizing hens with antigens. IgY is functionally equivalent to mammalian lgG. It is found in the serum of the chicken and is passed from the mother chicken to the embryo via the egg yolk, a process that results in a high concentration of IgY in the egg yolk. The objective of this study was to evaluate the efficacy of specific IgY against enterotoxigenic Escherichia coil (ETEC) K99, Salmonella Typhimurium and Salmonella Choleraesuis that cause porcine bacterial diseases. To prepare specific IgY, Hy-Line Brown chickens were vaccinated with killed vaccine complex including E. coil 1(99, S. Typhimurium and S. Choleraesuis. The chicken egg yolk antibodies were purified from egg yolk by ammonium sulfate precipitation and the quality of the final preparation was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Titres of specific IgY in final preparations were measured by an enzyme-linked immunosorbent assay (ELISA). Antibody titers peaked at 3 weeks regardless the bacterial types and the similar patterns of immune response were observed for respective pathogens. In growth inhibitor test, specific IgY showed inhibitory effect on bacterial growth. After 0, 3, 6 and 12 hour of incubation with specific lgY (100 ag/nil, 250 /m1, 500 tig/inl), there was a significant decrease in the growth (A600nm) of E. coil K99, S. Typhimurium and S. Choleraesuis compared to nonspecific IgY and controls. In BALB/c mice, the effect of specific IgY (100 nig/kg, 250 mg/kg) on bacterial challenges was investigated by intramuscular injection and oral administration of bacteria. Mice treated with specific IgY showed high survival rate though there was no significant differences on blood biochemichal examinations between treated and untreated groups. These results indicate the potential of specific IgY for the treatment of porcine bacterial diseases caused by E. coil K99, S. Typhimurium and S. Choleraesuis.
|
|
|
KEYWORD
|
|
|
Immunoglobulin egg Yolk(lgY), Efficacy, Escherichia coil K99, Salmonella Typhimurium, Salmonella Choleraesuis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|